Place it as the punchline after a short setup clip that states the wrong assumption, then follow it with the Vine Boom Sound Button for emphasis. For a second version, put the Dun Dun Dun Sound Button before it instead.
About this sound
This 2.7-second Portuguese voice clip delivers the line 'Eu nao sou gay nao' in a firm male voice, with the repeated 'nao' giving it a stubborn, defensive edge. It feels funny and confrontational at once, like a character pushing back hard against a wrong assumption. The line comes from Ted 2.