Place it at the very end of a scene, after the setup has made the trick clear. Pair it with Okay Let's Go Meme just before the reveal to build the scheme, then let this clip close the moment. For a bigger fall, follow it with Nope Meme Sound when the other person reacts.
About this sound
This is a short clip of Stewie Griffin laughing, lasting just over two seconds, with his clipped, high-pitched cartoon voice bursting into amused giggles. It feels smug and mischievous, like someone quietly pleased with a clever scheme. The laugh comes from Family Guy, the animated series created by Seth MacFarlane that first aired in 1999, where Stewie is known for dry wit and exaggerated confidence.