Place it as the second scare in a short horror edit, after a warning cue sets up the moment and before the clip cuts to the player's reaction. Pair it with the FNAF jumpscare scream for a double scare, or follow the danger alarm meme sound with it when a plan falls apart on screen.
About this sound
This is the sharp, distorted scream sting that fires the moment Flumpty Bumpty, the egg character, catches the player in One Night at Flumpty's. Lasting just over two and a half seconds, it turns quiet tension into sudden shock, then nervous laughter. It comes from the fan-made horror game inspired by Five Nights at Freddy's, where the scare lands without warning.