Put it at the very end of a scare sequence, after a tense build and a fake safe moment, then cut straight to credits or a reaction shot. Pair it after the Danger Alarm Meme Sound for a warning that turns real, or follow it with the FNAF Jumpscare Scream for a second scare in quick succession.
About this sound
This is the short, harsh jumpscare burst from the classic mode of Five Nights at Treasure Island, a fan-made horror game built around surviving a haunted attraction after dark. It hits with sudden, distorted noise that feels startling and tense, the kind of shock that makes a quiet room jump. It works best as a pure scare moment rather than background sound.