Put it at the end of a short fail or reaction sequence, after the mishap has been shown, then follow it with a Ba Dum Tss Drum Sting for a second punchline, or swap in a Funny Ha Ha Laugh Sound if the moment needs a more human reaction.
About this sound
This is a short clip of Goofy's loose, wobbly cartoon laugh, lasting just under two seconds, with no words, just that familiar chuckle. It feels bright, silly and harmless, the kind of sound that makes a clumsy moment feel playful instead of embarrassing. It comes from Disney's Goofy, the long-running cartoon character whose voice and laugh became familiar through animated shorts, television and films.