Place it at the end of a fail or argument clip, after a short reaction such as Bruh, so the insult lands as the payoff. Follow it with Emotional Damage when the target needs one more hit, or open with Oh My God Bro Oh Hell Nah before the argument escalates.
About this sound
This is a roughly six-second shouted version of the phrase 'go fuck yourself', stretched out and delivered at full volume by a single voice. The delivery is aggressive and deliberately over the top, turning a blunt insult into something confrontational and darkly funny. It works best as a sudden, exaggerated put-down rather than a serious insult.