The line comes from real-time strategy game Warhammer 40,000: Dawn of War, released in 2004, where Imperial Guard Guardsmen units shout their battlefield barks when enemies appear. As a unit response it was meant to warn the player quickly, similar to an Enemy Spotted callout in other strategy and shooter games. Clips of Dawn of War barks later circulated on soundboards and in Warhammer fan edits, and this Chaos warning stayed popular because it captures the Guard's role as ordinary men facing cosmic horror.
About this sound
A Warhammer 40k Dawn of War Guardsman shouting that Chaos has been spotted, used in gaming edits for spotting an enemy or revealing a threat.
This is the frantic Imperial Guardsman alert from Warhammer 40,000: Dawn of War, a panicked shout that Chaos forces have been spotted. The voice is thin, urgent and slightly overwhelmed, exactly how a regular human soldier would sound when facing daemonic enemies. It lasts just a couple of seconds but lands instantly because the fear is genuine rather than heroic. Viewers hear it and immediately understand something far more powerful has arrived, which makes the clip funny when it is dropped onto ordinary situations where someone is hopelessly outmatched.