Place it immediately after the accusation line in your timeline, then follow it with a vine boom sound effect to underline the reveal, or swap that second hit for a dramatic dun dun dun when the joke needs a slower build.
About this sound
This clip is a repeated series of loud, exaggerated gulps lasting about fifteen seconds, with each swallow sounding bigger and more desperate than the one before it. It feels funny and tense at the same time, capturing pure panic, guilt and embarrassment in a way that makes an awkward pause feel instantly relatable and dramatic.