Place this clip after the visual reveal of the setback, then follow it with the sad meow song for a second deflated beat, or precede it with the emotional damage meme to set up the disappointment first.
About this sound
This clip is the main melody from Heaven Knows I'm Miserable Now played solo on melodica for about thirteen seconds, thin and breathy with a slight wobble. It feels plaintively sad yet lightly comic, turning real melancholy into a self-aware sigh. It comes from The Smiths' 1984 song written by Morrissey and Johnny Marr.