Place it just after an enemy spotted cue when the Guest notices the player, then follow it with a jumpscare scream for the next beat in the chase. Use it as the turning point in a montage, between the search and the pursuit that follows.
About this sound
This 1.2-second clip features the Guest from Hello Neighbor 2 making an angry, irritated sound, a brief vocal outburst from the game's unsettling neighbour figure. It feels tense and confrontational, like being caught somewhere you should not be, and it lands as a sudden warning. It comes from Hello Neighbor 2.