Place it at the payoff of a short edit, right after the visual reveal and before the reaction beat. Follow it with the chipmunk laugh for a sillier close, or put awkward crickets after a failed attempt to let the contrast do the work.
About this sound
This is a short six-second clip of a female voice hitting an extremely high, bright note in a pop-diva style. It feels playful and a little over-the-top, turning an ordinary moment into a dramatic high-note reveal that listeners instantly read as funny and showy.