Place it just before the reveal in an edit, then follow it with Hell Naw Dog for the disbelief and Coffin Dance Meme when the fail is final, keeping the pause as the turning point between setup and payoff.
About this sound
This bass-boosted 'Hold Up' clip runs for about 20 seconds, with a deep, heavy low end that dominates the sound and makes the pause feel deliberate. It feels tense and dramatic, like a warning before something big happens, and it works best when the moment needs weight rather than a quick laugh.