The line comes from Grand Theft Auto: Vice City, the 2002 Rockstar game set in a fictional 1980s Miami-inspired city. Its short voice clips have long circulated separately from gameplay as reaction sounds, shared in edits, streams and soundboards. This quote survived because the name Mario and the self-directed warning are easy to recognise out of context, even for viewers who have not played the game. It is now used less as a reference to a specific scene and more as a standalone cue for calming down.
About this sound
The Mario take it easy sound effect is a short GTA Vice City voice line where he tells himself to calm down, used for overreaction and stress memes.
This is a brief spoken line delivered in a tense, self-soothing tone, as if the speaker is trying to stop himself from panicking or losing his temper. The words come out quickly but deliberately, with a pause that makes the self-reminder feel both serious and funny. Because he is literally talking himself down, the clip creates an instant picture of someone overwhelmed by a small problem and dramatically coaching himself through it. That contrast between stress and forced calm is what makes the moment land as comedy rather than genuine advice in most edits.