Place it right after the bad suggestion is shown, so it becomes the turning point of the edit. Build tension first with Sudden Suspense, then let this clip answer, and follow it with No No Wait Wait when the other side keeps arguing.
About this sound
This almost nine-second clip pairs the spoken line 'I will not allow this' with music, giving a short refusal a dramatic, drawn-out weight. It feels firm, defiant and a little theatrical, like drawing a line in a moment that has gone too far. The music lifts the words beyond a plain no, so the refusal lands as a confident, meme-ready objection.