This clip appears to be an original voice sample rather than audio taken from a film, show or game. The published description credits the performance to Dr. Devil and notes that it was made using Audacity. Sounds like this spread through soundboards and short meme edits because they give creators a ready-made villain reaction without needing copyrighted dialogue from a bigger franchise. It stays useful because the line is simple, recognizable on first listen, and fits competitive jokes, prank endings and over-the-top reaction posts.