The clip comes from My Little Pony Friendship Is Magic, the animated series that premiered in 2010, where Pinkie Pie is the hyperactive party pony of Ponyville. Voiced by Andrea Libman, the character is known for breaking into songs, impressions and non sequiturs, so a sudden chicken announcement fits her random humour perfectly. As the show built a large online fandom, short Pinkie Pie lines were ripped for soundboards and meme edits. This one survived because calling someone a chicken is an old playground taunt, and her proud delivery makes it reusable far beyond the fandom.
About this sound
Pinkie Pie shouting I'm a chicken in a silly voice from My Little Pony, used as a funny sound effect for coward jokes and random meme edits.
This is Pinkie Pie doing her over the top chicken impression, proudly announcing that she is a chicken in a bright, squeaky voice before breaking into clucking energy. The delivery is fast, bouncy and completely unserious, which is exactly why it lands as a punchline. It turns an ordinary moment into a silly farmyard joke, making whoever it is aimed at sound cowardly, confused or just randomly absurd. The contrast between the confident declaration and the ridiculous claim gives editors a short, clear reaction that needs no context to understand and works even on mute captions.