Place it at the start of a wind-up, right before the attempt itself, then follow it with the Fail Sound Meme for a flop, or let the It Was At This Moment He Knew line set up the attempt first.
About this sound
This 0.3-second clip packs an inhale, a quick beat on the chest and a sharp exhale into one tiny cartoon gasp, with a squeaky, toy-like edge to the breathing. It feels comic and breathless, like someone winding up for a dramatic effort and letting it out all at once. It comes from the Donald Duck cartoon Donald's Off Day, released on 8 December 1944.