This sound is tied to The Binding of Isaac: Repentance, the major expansion for The Binding of Isaac: Rebirth released in 2021. Repentance became widely shared through gameplay videos, challenge runs, and meme edits made by the game's community. Short intro and menu-related audio from the series often gets reused because it instantly signals Isaac to players. It still shows up in clips because the game has a strong recognizable style and a large audience that understands the reference right away.