Place it at the end of a short refusal sequence, after a setup clip and before a reaction sound. Start with Bruh Soundboard Button for the disbelief, follow with this denial, then close with Emotional Damage Sound Button when the rejection sinks in.
About this sound
This short clip captures Jotaro Kujo repeating no three times before shouting a final, louder NO, his voice rising with each word across about three seconds. It feels frantic, stubborn and funny, like a refusal that keeps getting stronger instead of backing down. The line comes from JoJo's Bizarre Adventure, where Jotaro's blunt delivery turned it into a widely shared meme reaction.