Place it at the end of an over-explained segment as the final reaction, after a bruh sound for disbelief and before a meme sound button sting to close the bit for the audience.
About this sound
This is a short Portuguese voice clip lasting just under three seconds, with a man saying 'João Antônio, já entendi agora' in a flat, done-with-this tone. It feels exasperated and funny at the same time, like someone politely shutting down an explanation that has gone on far too long and making it clear the message landed.