Place this clip at the end of a short exchange, after the original funny comment and before your reaction shot. Follow it with the bruh meme sound for a deflated payoff, or use a fail sound meme button if the backtrack collapses completely.
About this sound
This is an 11-second voice clip of Joe Pesci angrily pressing someone on what they meant by calling him funny, repeating the question with rising disbelief. It feels tense, awkward and darkly comic, because a casual compliment suddenly sounds like a challenge nobody in the room knows how to answer safely.