Place it at the exact frame of the reaction, after a setup line and before a cutaway. For a bigger sequence, follow it with a scream meme variant for a second hit, or precede it with an oh my god meme reaction to build the shock in steps.
About this sound
A short two-second clip of a single loud scream, presented here as the Orange Scream. It feels sudden and over-the-top, giving listeners a jolt of surprise that turns comic, like a reaction far bigger than the moment that caused it.