Place it right after the questionable line in your edit, before any response, then follow with the Eh Eh Ehhhh Sound Button as a follow-up take, or switch to the Awkward Moment Sound Button if the scene needs to stall instead of answering.
About this sound
This is a 1.5-second voice clip of Kotomine Kirei letting out a drawn-out, questioning Ooouuu, rising as if he has just heard something worth probing. It feels curious and faintly unsettling, mixing genuine confusion with sly interest. The sound comes from Kirei, the priest antagonist in the Fate anime series.