Place it right at the reveal of a refused request, after the ask has been read on screen. Follow it with the SpongeBob Fail Sound Button for a deflated reaction shot, or precede it with the Dun Dun Dun Sound Button when building up to the answer.
About this sound
A very short spoken clip of the German line 'Leider nein, leider gar nicht' delivered in a flat, matter-of-fact voice. The double refusal turns a simple no into a total shutdown, giving listeners an amused, slightly awkward feeling of polite finality, like a door closing with no room left to argue.