This clip appears to be a user-generated soundboard entry rather than a line taken from a known film, show or viral video, with its file metadata pointing to a voice-changer tool. That makes its exact original recording difficult to trace, but its appeal is easy to understand: the squeaky, cat-like scream is instantly recognisable as comic panic. Shared through meme soundboards, it is reused by editors who need a very short, punchy reaction sound for fails, scares and absurd reveals, keeping it useful long after its first upload.