This clip appears to be a user-generated soundboard entry rather than a line taken from a known film, show or viral video, with its file metadata pointing to a voice-changer tool. That makes its exact original recording difficult to trace, but its appeal is easy to understand: the squeaky, cat-like scream is instantly recognisable as comic panic. Shared through meme soundboards, it is reused by editors who need a very short, punchy reaction sound for fails, scares and absurd reveals, keeping it useful long after its first upload.
About this sound
A short, high-pitched cat-like scream made with a voice changer, used in funny edits and reaction videos to exaggerate shock, panic or a sudden scare.
This is a very short, sharp scream that sounds like a small child imitating a startled cat, stretched and pitched up through a voice changer until it becomes squeaky, frantic and almost cartoonish. It lasts just over a second, opening with a sudden yelp and ending almost as quickly as it begins, which makes it feel abrupt and silly rather than frightening. In a video it lands as a burst of panic, turning a small fall, a jump scare or an unexpected reveal into something exaggerated and funny. The childlike, animal quality keeps the moment light, so viewers hear surprise and chaos without taking the scene seriously.