Place it right after the hit lands in an edit, then follow it with Ara Ara for an anime-style reply, or cut to Nope when the character refuses to take another hit.
About this sound
This is a 1.4-second voice clip of Lisa from Genshin Impact saying 'ittaaii', the Japanese word for 'it hurts', in a drawn-out tone. It feels playful and overdramatic, like a light complaint rather than real pain, which makes it easy to drop into a joke about a small sting or setback.