Place it near the end of a confused exchange, after the rambling setup and before the reaction shot, so the question resets the scene. Pair it with You What for a back-and-forth misunderstanding, then follow with What Are You Aiming At to keep the questioning going.
About this sound
This five-second voice clip from Littlest Pet Shop delivers the question 'What, are we talking about?' in a puzzled, slightly exasperated tone, as if the speaker has just lost the thread of a conversation. It feels playful and bemused rather than angry, with the bright delivery of the show carrying the humour. The line comes from the television series.