Place it in the middle of a reaction sequence, after the Bruh Sound Effect sets up the confusion and before the Game Show Buzzer Sound Effect closes the failed idea. That order gives the moment a clear setup, a silly middle and a firm finish.
About this sound
This 16-second clip is a single cartoon brain fart stretched, slowed and turned up loud, with a low raspberry that drags on far longer than expected. It feels deliberately silly and groan-worthy, turning a small lapse in thinking into a drawn-out joke. Meme editors remade the short gag in the slowed-and-loud style to make the awkward pause linger.