Place it immediately after the failed play or bad call, then follow it with a Vine Boom to underline the impact and a SpongeBob Fail to close the sequence on the mistake itself.
About this sound
This is a short, sharp spoken reaction clip built around a stunned 'what was that' outburst, delivered in a frustrated, incredulous tone over just a few seconds. It lands as pure disbelief, the sound of someone watching something unfair, absurd or completely unexpected happen in front of them and calling it out immediately, with no build-up and no softening.