Place it at the end of a reveal, after a short build-up, so it closes the scene instead of opening it. Start with Dun Dun Dun to signal something is about to go wrong, let the laugh deliver the twist, then finish with Vine Boom for a hard cut to the reaction.
About this sound
The clip is a fourteen-second puppet laugh, with several high, exaggerated voices cackling over each other in a theatrical, jeering chorus. It feels sinister and gleeful at once, like a joke shared at someone else's expense, and it lands best as a dark, knowing reaction rather than a light giggle.