Put it near the start of a horror reaction edit, just before the moment the scare arrives, then follow it with a jumpscare scream for contrast and a danger alarm to mark the next scare.
About this sound
This is a short Ghostface voice line from Mortal Kombat 1, lasting under three seconds, in which the masked killer says he has never seen a scary movie. The deadpan admission feels funny and slightly unsettling because it flips the horror villain into a clueless outsider. It comes from the game's Ghostface dialogue.