The sound comes from My Little Pony, the long-running animated franchise best known in meme culture through Friendship Is Magic, which premiered in 2010. Clips of the ponies screaming have circulated for years as reaction audio on forums, video edits and soundboards, detached from their original episodes. Its appeal is the contrast between the show's cute, colourful style and a completely outsized scream, making it perfect shorthand for melodramatic fear. That contrast has kept it in rotation alongside other cartoon scream memes long after the series ended.
About this sound
The My Little Pony screaming sound is a high-pitched cartoon pony scream from the animated series, used for funny panic and overreaction moments in edits.
This clip is a long, shrill cartoon scream in a high pony voice that keeps rising and wavering as if the character has spotted something terrifying. It sounds bright, squeaky and deliberately over the top, with the pitch stretching out for several seconds before fading. The exaggeration is what makes it funny rather than scary, turning a moment of shock into instant comedy. Viewers recognize it immediately as a panic reaction, and the longer it goes on the more absurd the situation feels, which is why editors let it play out in full.