The sound comes from My Little Pony, the long-running animated franchise best known in meme culture through Friendship Is Magic, which premiered in 2010. Clips of the ponies screaming have circulated for years as reaction audio on forums, video edits and soundboards, detached from their original episodes. Its appeal is the contrast between the show's cute, colourful style and a completely outsized scream, making it perfect shorthand for melodramatic fear. That contrast has kept it in rotation alongside other cartoon scream memes long after the series ended.