This sound is associated with Kumoko from Kumo Desu ga, Nani ka? and, based on the provided source note, was taken from a light novel TV commercial rather than a longer scene. The series comes from the work by Baba Okina, and clips of Kumoko's expressive voice spread easily in anime meme edits because her reactions are exaggerated but still cute. Short sounds like this stay popular because they fit many situations, especially edits built around disbelief, embarrassment, or a playful rejection.