Place this clip at the end of a short reaction sequence, after the mistake has already played out for the viewer. Follow a failed attempt with the bruh sound effect for the reaction, then use this denial to close it, or precede it with emotional damage meme for a bigger fallout before the shutdown.
About this sound
A sub-second voice clip of Brazilian streamer Smzinho saying the Portuguese phrase "não negão" in a fast, clipped delivery. The firm but amused emphasis makes it land as a funny, instant shutdown, the kind of blunt joke denial listeners recognise immediately without any argument or explanation needed.