Place it as the final beat after the setup clip, so the accusation lands as the punchline. Pair it with a laugh track straight afterwards, and put a dramatic sting just before it to set up the callout.
About this sound
This is a very short voice clip, just over two seconds long, built around a blunt spoken accusation delivered with sharp, mocking attitude. It feels confrontational and unserious at once, turning a sudden interruption into an instant roast. The line comes from The Boondocks, the adult animated television series whose short quoted lines became widely reused reaction audio online.