The clip comes from the US version of The Office, in the 2008 episode Frame Toby, where Michael Scott discovers that Toby Flenderson has returned from Costa Rica to HR. Michael's long standing hatred of Toby turns a normal office moment into pure panic, and fans clipped the scream as a reaction and GIF across forums and social media. It stayed popular because almost any unwanted return, sequel, update or person can be framed the same way, making the exaggerated horror instantly relatable.
About this sound
Michael Scott screaming no God please no from The Office when Toby returns. People use this reaction sound for dread, bad news and unwanted surprises.
This is Michael Scott's panicked outburst from The Office, starting with a desperate no God please no and building into a long, drawn out scream. His voice cracks from shocked whisper to full volume horror as if he has seen the worst possible person walk back into the room. The over the top delivery makes a minor workplace annoyance feel like a catastrophe. Viewers instantly feel the dread and secondhand embarrassment, which is why the clip works even without the picture, the desperation in his voice tells the whole story by itself.