Place it immediately after the annoying moment peaks, not before the audience understands the pestering. Open with Sudden Suspense to build the tension, then let this clip answer it, and follow with No No Wait Wait when the other person tries to backtrack and keep the argument going.
About this sound
This is a short spoken Spanish phrase, lasting just over two seconds, delivered as a blunt telling-off to someone who will not stop. It carries a fed-up, exasperated tone that turns irritation into a joke, making the listener laugh at the speaker's impatience rather than feel attacked. It works as a quick, relatable complaint for everyday annoyance.