Place it immediately after the unfair act is shown in the edit, following No No Wait Wait as the first objection, then let Sad Violin follow it for a mock-sympathetic finish.
About this sound
This is a short Spanish voice clip, just over two seconds long, in which a speaker delivers the line 'No se vale lo que me has hecho' with a wounded, reproachful tone. It lands as a direct accusation of unfair treatment, carrying hurt and indignation rather than anger, and it works as a pointed reply when someone has crossed a line.