Place it at the very end of a joke setup, as the payoff that closes the bit, then move straight to the next scene without adding another reaction. For a bigger flop, follow it with the SpongeBob Fail sound, and use the Vine Boom sound earlier to set up the joke before the letdown.
About this sound
This is a short, deadpan spoken line saying 'not funny, didn't laugh,' delivered in a flat, unimpressed tone that lands like a verdict on a bad joke. The clip feels dry, awkward and deliberately harsh, turning a failed punchline into the joke itself. Its bluntness makes it funny precisely because it refuses to laugh, leaving the silence after it to do the work.