Place it right after the reveal in a reaction sequence, then follow with the Owen Wilson Wow Sound Button for a second reaction beat, or precede it with the Drama Button Soundboard when building toward a bigger group reaction in comedy edits.
About this sound
This clip is a short, wide-eyed 'O' reaction, the kind of stunned vocal gasp that matches a character caught completely off guard. It feels funny and awkward in equal measure, turning a tiny surprise into an exaggerated moment. It is tied to Thomas the Tank Engine through its TTTE tag.