Place it at the peak of a failed stunt, then follow it with Nelson's Ha-Ha! for a second laugh, or lead in with Cartoon Slip so the fall sets up the reaction.
About this sound
This 2.5-second clip pairs Riley the Clown's startled 'oh my god' exclamation with a clown honk in one short burst. The mix of genuine shock and cartoon silliness makes it feel playful and absurd, like a joke landing a beat too loudly.