Place it at the start of the disaster, then follow it with Nope Meme Sound to shut the idea down, or build to No God Please No for a bigger reaction. It also works after Danger Alarm Meme Sound when the warning has already come too late.
About this sound
This is a 2.6-second vocal reaction saying 'oh no' in a strained, theatrical delivery. It feels funny and tense at once, turning a small mistake into a bigger, more dramatic disaster. The clip is associated with JoJo's Bizarre Adventure, which gives it that anime flavour creators reach for in fail edits and sudden reveals.