Place it as the payoff after a warning shot in your edit, not as an opener. Lead in with No No Wait Wait when someone ignores advice, then let this clip land, and follow with Nelson's Ha-Ha! for the group reaction afterward.
About this sound
A short, loud male voice shouts the warning 'oh now you poked the bear' in a 2.3-second burst taken from The Morning Invasion segment 'The Boys Make a Bet'. It feels confrontational and funny at once, like a playful threat that says a line was crossed and consequences are about to start.