There is no single documented source for this short 'ooohhh no' clip, which is part of why it works so widely as a reaction sound. Short vocal reactions like this circulate on soundboards, video edits and livestreams because they are instantly understood without context, and creators reuse them whenever a joke needs a quick beat of disbelief or disappointment. Its length, just over a second, makes it easy to drop into memes, gaming highlights and comedy edits, keeping it in regular use long after many longer clips fade.