Place it just before the reveal in an edit, so the checkup sets up the bad news and the next sound delivers the reaction. Follow it with Law and Order Dun Dun for a dramatic turn, or with SpongeBob Sad Song when the result is meant to feel deflating rather than shocking.
About this sound
Otis' Checkup is a television clip from Dead Cow Walking built around a checkup scene. The moment lands as awkward and uneasy, the kind of humour that comes from bad news delivered in a calm setting. The comedy sits in the contrast between routine procedure and a result nobody wanted to hear, leaving the listener unsure whether to laugh or wince.