Place it at the reaction beat after the mistake lands, then follow it with the Anime Ahhh sound for a second reaction layer, or switch to the Screaming Sheep sound when you want the gag to escalate into absurd territory instead of ending.
About this sound
A 4.5-second anime-style clip of a young female voice letting out a loud, sustained scream. It feels frantic and over-the-top, turning a small scare or surprise into a big, funny meltdown that listeners instantly read as panic for everyone listening.