Use it as the second beat in a short gag: open with the Goofy Yell Sound Effect for the setup, let the quack answer it, then close with the Shocked Meme Sound Effect for the reaction. That order keeps the joke moving from setup to reaction without crowding the edit.
About this sound
This is a single 0.2-second duck quack, a sharp animal call with a bright, cartoony edge that cuts through straight away. It feels cheeky and instantly funny, like a small joke arriving before anyone has time to react. Because it needs no setup, it works as a quick nonverbal reaction for a fail, a strange choice or a random moment on screen.