Put it at the end of a setup clip, right where a decision is made, then follow it with Okay Let's Go to move straight into the action. For a back-and-forth exchange, place Nope just before it so the change of mind lands harder.
About this sound
This clip is Rammus, the armoured champion from League of Legends, answering with one French “oui” in a deep voice that barely rises above a murmur and finishes in under two seconds. That unhurried, matter-of-fact yes feels stern and oddly comic, as if the decision was settled long before anyone asked. It comes from the French voice-over of League of Legends, where Rammus is known for brief replies.