The phrase is closely associated with Scooby-Doo, the talking Great Dane from the long-running Scooby-Doo cartoon franchise that began in 1969. In the show, it is his nervous way of saying 'uh-oh' when the gang stumbles into danger or a mystery takes an unexpected turn. Because the sound is short, instantly recognisable and easy to read without context, it travelled from television clips into online memes, reaction videos and soundboards, where it now signals that trouble has just been spotted.