Place it in the middle of a visual gag, after It Was At This Moment He Knew sets up the realisation and just before the character disappears from view. If the escape fails, follow it with Fail Sound Meme for the catch; if it succeeds, close with a cheer instead.
About this sound
This is a split-second burst of tiny, hurried cartoon footsteps matched to bright, rising xylophone notes. It feels light, frantic and sneaky, like someone darting away just before being spotted, with a playful rush that turns a quick escape into a joke. It comes from the 1942 Donald Duck cartoon Donald Gets Drafted.